goat anti-monkey iga-alpha chain-specific biotin  (Alpha Diagnostics)

Journal: iScience

Article Title: Intranasal murine pneumonia virus-vectored SARS-CoV-2 vaccine induces mucosal and serum antibodies in macaques

doi: 10.1016/j.isci.2023.108490

Figure Lengend Snippet:

Article Snippet: After incubating for 1 hour at RT on a rotating shaker, plates were washed 3 times with 250 μl washing buffer, and incubated on a rotating shaker for another 20 min. 100 μl per well containing a mix of secondary goat anti-monkey IgG(H+L)-horseradish peroxidase (HRP) conjugated antibody (ThermoFisher, cat# PA1-84631) at 1:10,000 dilution and goat anti-monkey IgA-alpha chain specific Biotin (Alpha Diagnostic International, cat# 70049) at 1:5,000 dilution in dilution buffer, was added, and plates were incubated for 1 h. After incubation with the secondary antibody mix, plates were washed three times and 100 ml per well of Streptavidin-Europium (PerkinElmer, cat# 1244-360) diluted at 1:2,000 in PBS+ 0.2% IGEPAL CA-630 was added.

Techniques: Diagnostic Assay, Expressing, Recombinant, Fluorescence, Mutagenesis, Silver Staining, Clinical Proteomics, Generated, Software

Journal: Cell Reports Medicine

Article Title: Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infection

doi: 10.1016/j.xcrm.2022.100528

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Article Snippet: Anti-Monkey IgA (α-chain specific)-Biotin antibody produced in goat , Sigma Aldrich , Cat# SAB3700761.

Techniques: Produced, Recombinant, Bradford Protein Assay, Purification, Ligation, Sequencing, Expressing, Luciferase, Plasmid Preparation, Clone Assay, Software, Enzyme-linked Immunosorbent Assay, Staining, Luminex